Multiple choice question for engineering
1. If expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?
a) The extract in which the host DNA constructs are incubated is capable of translation only
b) The extract in which the host DNA constructs are incubated is capable of transcription only
c) The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc.
d) Non radiolabelled amino acids are used
2. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______
a) coupled, linked
b) linked, coupled
c) grouped, separate
d) grouped, individual
3. If translation and transcription are carried out separately, then what is added to carry out translation?
a) DNA polymerase
b) RNA polymerase
c) Taq polymerase
d) Tfl polymerase
4. If the screening of the DNA constructs is carried out in pooled batches then it takes less time. Is the given statement true or false?
5. Choose the incorrect statement for open reading frame.
a) It is helpful in deciding whether the clone is correct or not
b) It consists of an initiation codon
c) It consists of termination codon
d) The presence of introns doesn’t affects the open reading frame
6. Introns are ________ in nature and the sequence is _______ than exons.
a) coding, less random
b) non-coding, less random
c) non-coding, more random
d) coding, more random
7. If the genome size is small, then sequencing of the entire genome is:
c) independent of the size of genome
d) not possible
8. Choose the correct statement with respect to bacterial genome.
a) They are easy to sequence
b) They are difficult to sequence
c) Methods such as transcription and translation are satisfactory
d) Only transcription can be carried out
9. For identification of genes that are expressed under special conditions, _______ libraries are used.
10. In ______ organisms, firstly the gene is looked into model organisms.
c) both eukaryotic and prokaryotic
d) large sized
1. If overexpression of proteins takes place, they become toxic at times. In such a case, _______ colonies are recovered after ______ with the recombinant plasmid.
a) few, translation
b) few, transformation
c) no, transformation
d) no, translation
2. Ribosome binding site in E.coli is composed of which sequence primarily?
3. Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.
4. Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. Is the given statement true or false?
5. If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.
a) N terminal
b) C terminal
c) both N & C terminal
6. Proteins synthesized by carrying out translation of the vector region and continuing it in the insert region is called as:
a) hybrid protein
b) fusion protein
c) combination protein
d) insert protein
7. It is also possible to generate a fusion protein in which ______ is encoded by the vector.
a) N terminal
b) C terminal
c) both N and C terminal
d) middle region
8. Which of the following is present in E. coli rrnB operon for rRNA?
a) Initiation codon
b) Termination codon
c) Both initiation and termination codon
d) Both are absent
9. Termination codon is required for stopping high levels of transcription of other region such as _______ which might interfere with ____ of the vector.
a) origin, replication
b) end, replication
c) end, stability
d) origin, stability
10. In fusion proteins the reading frame in vector is out of phase with the insert. Is the given statement true or false?
1. A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated?
a) Filtration and centrifugation
b) Filtration or centrifugation
c) Allowing to sediment
d) By passing through silica column
2. Another method of DNA recovery is termed as freeze-squeeze. The correct statement for it is?
a) The slice containing DNA is cut and frozen into liquid oxygen
b) Freezing doesn’t have any effect on the DNA structure
c) After freezing centrifugation is carried out through glass wool plug
d) The substance used for centrifugation allows the gel to pass through but the liquid is retained
3. If further electrophoresis is used for recovery of DNA from gels, the method is termed as:
a) secondary electrophoresis
b) recovery electrophoresis
c) denaturing electrophoresis
4. The DNA is sliced and is placed into the dialysis tube containing buffer. Further electrophoresis is performed. After it, the DNA moves out of the gel but is retained by buffer. The given statement is true or false?
5. UV shadowing is used at times for visualising the DNA. Which of the statements is correct for it?
a) In this method, the DNA bands are visualized because they fluoresce under UV
b) The DNA bands won’t fluoresce but will absorb the UV light
c) It is not suitable for visualizing single stranded molecules
d) It is less preferred over use of ethidium bromide
6. The greatest separation is obtained in which portion of the gel?
a) Lower portion where the anode is
b) Lower portion where the cathode is
c) The separation is uniform all over
d) It varies according to the quantity of the size of the molecules to be separated
7. Which of the statements is correct for non uniform separation obtained other than the conventional one?
a) It can be obtained via applying strong field towards the cathode
b) It can be obtained via applying same field on both the sides
c) The separation is greater on the side of stronger field
d) Its is greater towards the weaker field
8. How can gradient in the field strength be obtained?
a) It can be obtained via varying the amount of current, keeping the resistance constant
b) It can be obtained via varying the amount of resistance, keeping the current constant
c) Both can be varied, but resistance is having more effect
d) Buffer-gradient gels can be used with decreasing concentration of buffer at the bottom.
9. If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?
a) Wedge gels
b) Buffer-gradient gels
c) Pulse field gels
d) Varying the amount of agarose, will carry out the separation
10. Which of the following statement is correct for the method of separation of large molecules?
a) The large DNA molecules can pass through the matrix in any direction, not necessarily in the direction parallel to the movement
b) The separation can be carried out if the fields are at right angles to each other
c) The time of pulse should be less than that of the reorientation time
d) Separation is not of the megabase level
1. There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding, ______ and ______ formation.
a) solubility, phosphodiester bond formation
b) insolubility, phosphodiester bond formation
c) solubility, disulphide bond formation
d) insolubility, disulphidebond formation
2. A short peptide region fused to a protein of interest is known as:
3. Glutathione-S-Transferase (GST) enzyme is used for conjunction of Glutathione molecules and is having a protective function in many organisms. Is the given statement true or false?
4. Maltose binding protein is the product of ______ gene in E.coli and located in ______
a) malE, nucleus
b) malD, nucleus
c) malE, periplasmic space
d) malD, periplasmic space
5. Thioredexin protein contains two _______ residues.
6. Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.
a) calcium ions
b) nickel ions
c) iron ions
d) fluorine ions
7. Pel B protein is produced in plants and helps in degradation of ______
b) plasma membrane
c) cell wall
8. His tagged proteins can be eluted using EDTA or a pH gradient from the matrix. Is the given statement true or false?
9. Enterokinase is an intestinal enzyme that converts _______ to ________
a) pepsinogen, pepsin
b) pepsin, pepsinogen
c) trypsinogen, trypsin
d) trypsin, trypsinogen
10. Cyanogen bromide is used for cleavage of junctions. It cleaves after ________ residues.
d) phenolic acid
1. Gel electrophoresis separates nucleic acid molecules based on:
a) charge on molecules
b) size of the molecules
c) nature of the molecules i.e. whether DNA or RNA
d) chemical properties of the nucleic acids
2. The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to number of:
a) sugar molecules
b) nitrogenous bases
c) phosphate groups
d) both phosphate group and sugar molecules
3. Force is defined as mass per unit acceleration. As the number of phosphate molecules increase, the charge also increases which increases the force required. The acceleration is dependent of the size of the molecules. The given statement is true or false?
4. Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?
d) Supercoiled and circular will move at same speed and faster than nicked
5. How is the size of molecules under analysis measured?
a) By measuring the distance moved through a ruler
b) By measuring the amount of visualising dye used
c) By running a standard molecule, whose size is known in parallel
d) There is no exact criterion for doing so
6. Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels?
a) Agarose is a polysaccharide which is obtained from red algae
b) Agarose is a polysaccharide obtained from fungus
c) It is composed of glucose residues
d) It is obtained via dissolving in it water by boiling in water and then cooling it
7. Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?
a) They are obtained via polymerization between acrylamide and bis-acrylamide
b) The components added for initiating polymerization are ammonium persulphate and TEMED
c) It is casted in horizontal and flat trays
d) TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking
8. If the amount of agarose added is more, the molecular under analysis should have following characteristsic:
a) small size
b) large size
c) size has no relation with the amount of agarose
d) the amount of molecules under analysis matters
9. In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?
c) The routine agarose gel
d) The routine polyacrylamide gel
10. At times, a specific fragment of the molecules of DNA which are analyzed needs to be separated. One of the methods is digesting the gels simply. Which of the following statement is not correct in with respect to it?
a) The gelling temperature has an important role to play
b) For higher gelling temperatures, digestion of DNA is done via addition of agarase or some chaotropic agents
c) For lower gelling temperatures, simply slicing out of target DNA is done which is followed by melting
d) The DNA can be extracted via addition of sodium or any positively charged group